Composite

Part:BBa_K2888002:Design

Designed by: SBS_SH_112144   Group: iGEM18_SBS_SH_112144   (2018-10-06)


fused lysozyme gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 27
    Illegal NheI site found at 50
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We replace unexpected restriction site with corresponding code


Source

This cyanophage lysozyme discovered by Heideburg et al in Yellowstone National Park {Heideburg:2009hb}. And we use standard, common constitutive promoter , BBa_B0034, RBS BBa_J23100, as well as double terminator BBa_B0015.

References